CAS:80498-17-5,EcoR I

英文名称：EcoR I

其他名称：限制性内切酶EcoR I

CAS号：80498-17-5

级别：BR

来源：Recombinant E. coli strain that carries the cloned ecoRIR gene from Escherichia coli.

保存条件：10 mM potassium phosphate (pH 7.4 at 25°C), 300 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.2 mg/mL BSA, 0.15% TrIT(http://www.maoyihang.com/sell/l_25/)on X-100 and 50% glycerol.

缓冲液Optimal buffer(10X)：500 mM Tris-HCl (pH 7.5), 100 mM MgCl2, 1000 mM NaCl, 0.2% Triton X-100, 1 mg/mL BSA.

Ligations：After 10-fold over digestion with enzyme 95% of the  fragments can be ligated and recut.

Methylation Effects：Not dam methylation-sensitive, not dcm methylation-sensitive. CpG methylation-sensitive

Campatible Ends：Xap I, Mun I, Tas I, Acs I.

CAS:80498-17-5,EcoR I 使用说明：High enzyme concentration and using of nonoptimal buffer may result in star activity. Do not use BSA for long incubation. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.

性状(以下信息供参考)：液体

用途：本品供科研，不得用于其它用途。

保存：-20℃